AccScience Publishing / TD / Volume 3 / Issue 1 / DOI: 10.36922/td.2290
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ORIGINAL RESEARCH ARTICLE

Profiling energy metabolism in normal bladder tissue and non-muscle-invasive bladder cancer cases of different histological grades

Guilherme Prado Costa1,2 ,  Petra Karla Böckelmann1 ,  Renato Prado Costa2 ,  Carlos Hermann Schaal2 ,  Fernando César Sala2 ,  André Pereira Vanni2 ,  Leandro Luiz Lopes de Freitas3 ,  João Carlos Cardoso Alonso1,4 ,  Gabriela Cardoso de Arruda Camargo1 ,  Gabriela de Oliveira1 ,  Bianca Ribeiro de Souza5 ,  Athanase Billis3 ,  Wagner José Fávaro1*
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1 Laboratory of Urogenital Carcinogenesis and Immunotherapy (LCURGIN), Universidade Estadual de Campinas (UNICAMP), Campinas City, São Paulo State, Brazil
2 Department of Urology, Amaral Carvalho Hospital (HAC), Jaú City, São Paulo State, Brazil
3 Department of Pathology, Medical School, Universidade Estadual de Campinas (UNICAMP), Campinas City, São Paulo State, Brazil
4 Department of Urology, Paulínia Municipal Hospital, Paulínia City, São Paulo State, Brazil
5 Department of Obstetrics and Gynecology, OVCARE, University of British Columbia, Vancouver, British Columbia, Canada
Tumor Discovery 2024, 3(1), 2290 https://doi.org/10.36922/td.2290
Submitted: 22 November 2023 | Accepted: 9 January 2024 | Published: 19 March 2024
© 2024 by the Author (s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Bladder cancer (BC) stands as the second most common urinary tract malignancy. Recent years have witnessed a growing interest in investigating energy metabolism to help with better understanding the energy sources harnessed by tumor cells. The aims of the present study are to feature and compare cell energy metabolism profiles among different histological grades of non-muscle-invasive BC (NMIBC) by adjusting their bioenergetic cellular indexes based on the specific tumor types. Forty urinary bladder tissue samples from patients both with and without a diagnosis of urothelial lesions were collected. Subsequently, samples were categorized into four groups comprising ten samples each, namely: normal (no urothelial lesions) group, low-grade pTa group, high-grade pTa group, and high-grade pT1 group. These tissue samples were examined by means of immunohistochemistry and Western blotting to assess proteins involved in cell energy metabolism. Based on the current findings, the normal and low-grade pTa groups presented clear preference for the oxidative phosphorylation pathway; consequently, they recorded high bioenergetic cellular index. On the other hand, both the high-grade pTa and pT1 groups presented proclivity towards the glycolytic pathway. These observations, mainly those associated with the bioenergetic cellular index, hold promising clinical relevance in the management of BC. Given the often aggressive and potentially debilitating nature of treatments applied to this neoplasia type, the current study offers invaluable insights on this topic and emphasizes changes in the bioenergetic cellular index at different NMIBC grades, which could serve as potential markers for both the diagnosis and prognosis of NMIBC patients.

Keywords
Bladder cancer
Energy metabolism
Bioenergetic cellular index
Cancer metabolism
Funding
This research was funded by the São Paulo Research Council (FAPESP grant numbers: 2014/11866-1; 2014/12047-4; 2018/10052-1; 2020/03419-6), and the Brazilian National Council for Scientific and Technological Development (CNPq grant numbers: 552120/2011-1; 312396/2021-0).
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Conflict of interest
The authors declare that they have no competing interests.
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Tumor Discovery, Electronic ISSN: 2810-9775 Published by AccScience Publishing
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