Tracking of transplanted neural stem cells labeled with superparamagnetic iron oxide in ischemic stroke

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Abstract

Clinical treatments for ischemic stroke are limited. At present, neural stem cell (NSC) therapy is considered a promising method for treating ischemic stroke. However, the behavior of transplanted NSCs remains ambiguous. Herein, we report the feasibility of superparamagnetic iron oxide labeling for long-time magnetic resonance imaging to track transplanted NSCs in a transient middle cerebral artery occlusion model. According to our studies, in vivo monitoring of transplanted NSCs during ischemic stroke in the current work may provide another insight into the tracking strategy of transplanted NSCs, thereby advancing NSC-based therapy toward clinical application.

Keywords

Neural stem cell

Ischemic stroke

Superparamagnetic iron oxide

Magnetic resonance imaging

Tracking

Funding

National Natural Science Foundation of China

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest.

References

[1]

Datta A, Sarmah D, Mounica L, et al., 2020, Cell death pathways in ischemic stroke and targeted pharmacotherapy. Transl Stroke Res, 11(6): 1185–1202. https://doi.org/10.1007/s12975-020-00806-z

[2]

Herpich F, Rincon F, 2020, Management of acute ischemic stroke. Crit Care Med, 48(11): 1654–1663. https://doi.org/10.1097/CCM.0000000000004597

[3]

Tao T, Liu M, Chen M, et al., 2020, Natural medicine in neuroprotection for ischemic stroke: Challenges and prospective. Pharmacol Ther, 216: 107695. https://doi.org/10.1016/j.pharmthera.2020.107695

[4]

Wang F, Tang H, Zhu J, et al., 2018, Transplanting mesenchymal stem cells for treatment of ischemic stroke. Cell Transplant, 27(12): 1825–1834. https://doi.org/10.1177/0963689718795424

[5]

Jiang XC, Xiang JJ, Wu HH, et al., 2019, Neural stem cells transfected with reactive oxygen species-responsive polyplexes for effective treatment of ischemic stroke. Adv Mater, 31(10): e1807591. https://doi.org/10.1002/adma.201807591

[6]

Wechsler LR, Bates D, Stroemer P, et al., 2018, Cell therapy for chronic stroke. Stroke, 49(5): 1066–1074. https://doi.org/10.1161/STROKEAHA.117.018290

[7]

Bai Y, Ren H, Bian L, et al., 2022, Regulation of glial function by noncoding RNA in central nervous system disease. Neurosci Bull. https://doi.org/10.1007/s12264-022-00950-6

[8]

Chen J, Venkat P, Zacharek A, et al., 2014, Neurorestorative therapy for stroke. Front Hum Neurosci, 8: 382. https://doi.org/10.3389/fnhum.2014.00382

[9]

Hamblin MH, Lee JP, 2021, Neural stem cells for early ischemic stroke. Int J Mol Sci, 22(14): 7703. https://doi.org/10.3390/ijms22147703

[10]

Ding DC, Lin CH, Shyu WC, et al., 2013, Neural stem cells and stroke. Cell Transplant, 22(4): 619–630. https://doi.org/10.3727/096368912X655091

[11]

Bernstock JD, Peruzzotti-Jametti L, Ye D, et al., 2017, Neural stem cell transplantation in ischemic stroke: A role for preconditioning and cellular engineering. J Cereb Blood Flow Metab, 37(7): 2314–2319. https://doi.org/10.1177/0271678X17700432

[12]

Wang G, Han B, Shen L, et al., 2020, Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke. EBioMedicine, 52: 102660. https://doi.org/10.1016/j.ebiom.2020.102660

[13]

Tong L, Zhao M, Zhu S, et al., 2011, Synthesis and application of superparamagnetic iron oxide nanoparticles in targeted therapy and imaging of cancer. Front Med, 5(4): 379–387. https://doi.org/10.1007/s11684-011-0162-6

[14]

Kim SJ, Lewis B, Steiner MS, et al., 2016, Superparamagnetic iron oxide nanoparticles for direct labeling of stem cells and in vivo MRI tracking. Contrast Media Mol Imaging, 11(1): 55–64. https://doi.org/10.1002/cmmi.1658

[15]

Boese AC, Le QS, Pham D, et al., 2018, Neural stem cell therapy for subacute and chronic ischemic stroke. Stem Cell Res Ther, 9(1): 154. https://doi.org/10.1186/s13287-018-0913-2

[16]

Wang P, Ma S, Ning G, et al., 2020, Entry-prohibited effect of kHz pulsed magnetic field upon interaction between SPIO nanoparticles and mesenchymal stem cells. IEEE Trans Biomed Eng, 67(4): 1152–1158. https://doi.org/10.1109/TBME.2019.2931774

[17]

Keselman P, Yu EY, Zhou XY, et al., 2017, Tracking short-term biodistribution and long-term clearance of SPIO tracers in magnetic particle imaging. Phys Med Biol, 62(9): 3440–3453. https://doi.org/10.1088/1361-6560/aa5f48

[18]

Bashir MR, Bhatti L, Marin D, et al., 2015, Emerging applications for ferumoxytol as a contrast agent in MRI. J Magn Reson Imaging, 41(4): 884–898. https://doi.org/10.1002/jmri.24691

[19]

Chen B, Sun J, Fan F, et al., 2018, Ferumoxytol of ultrahigh magnetization produced by hydrocooling and magnetically internal heating co-precipitation. Nanoscale, 10(16): 7369–7376. https://doi.org/10.1039/c8nr00736e

[20]

Hu Y, Li D, Wei H, et al., 2021, Neurite extension and orientation of spiral ganglion neurons can be directed by superparamagnetic iron oxide nanoparticles in a magnetic field. Int J Nanomedicine, 16: 4515–4526. https://doi.org/10.2147/IJN.S313673

[21]

Wang Z, Xu P, Chen B, et al., 2018, Identifying circRNA-associated-ceRNA networks in the hippocampus of Abeta1- 42-induced Alzheimer’s disease-like rats using microarray analysis. Aging (Albany NY), 10(4): 775–788. https://doi.org/10.18632/aging.101427

[22]

Wu F, Han B, Wu S, et al., 2019, Circular RNA TLK1 aggravates neuronal injury and neurological deficits after ischemic stroke via miR-335-3p/TIPARP. J Neurosci, 39(37): 7369–7393. https://doi.org/10.1523/JNEUROSCI.0299-19.2019

[23]

Bai Y, Zhang Y, Han B, et al., 2018, Circular RNA DLGAP4 ameliorates ischemic stroke outcomes by targeting miR-143 to regulate endothelial-mesenchymal transition associated with blood-brain barrier integrity. J Neurosci, 38(1): 32–50. https://doi.org/10.1523/JNEUROSCI.1348-17.2017

[24]

Shi Y, Zhang L, Pu H, et al., 2016, Rapid endothelial cytoskeletal reorganization enables early blood-brain barrier disruption and long-term ischaemic reperfusion brain injury. Nat Commun, 7: 10523. https://doi.org/10.1038/ncomms10523

[25]

Zhao J, Zhang Z, Xue Y, et al., 2018, Anti-tumor macrophages activated by ferumoxytol combined or surface-functionalized with the TLR3 agonist poly (I: C) promote melanoma regression. Theranostics, 8(22): 6307–6321. https://doi.org/10.7150/thno.29746

[26]

Zakrzewski W, Dobrzynski M, Szymonowicz M, et al., 2019, Stem cells: Past, present, and future. Stem Cell Res Ther, 10(1): 68. https://doi.org/10.1186/s13287-019-1165-5

[27]

Kim HY, Kim TJ, Kang L, et al., 2020, Mesenchymal stem cell-derived magnetic extracellular nanovesicles for targeting and treatment of ischemic stroke. Biomaterials, 243: 119942. https://doi.org/10.1016/j.biomaterials.2020.119942

[28]

Sonntag KC, Song B, Lee N, et al., 2018, Pluripotent stem cell-based therapy for Parkinson’s disease: Current status and future prospects. Prog Neurobiol, 168: 1–20. https://doi.org/10.1016/j.pneurobio.2018.04.005

[29]

Okano H, Yasuda D, Fujimori K, et al., 2020, Ropinirole, a new ALS drug candidate developed using iPSCs. Trends Pharmacol Sci, 41(2): 99–109.https://doi.org/10.1016/j.tips.2019.12.002

[30]

Reya T, Morrison SJ, Clarke MF, et al., 2001, Stem cells, cancer, and cancer stem cells. Nature, 414(6859): 105–111. https://doi.org/10.1038/35102167

[31]

Rikhtegar R, Pezeshkian M, Dolati S, et al., 2019, Stem cells as therapy for heart disease: iPSCs, ESCs, CSCs, and skeletal myoblasts. Biomed Pharmacother, 109: 304–313. https://doi.org/10.1016/j.biopha.2018.10.065

[32]

Chrostek MR, Fellows EG, Crane AT, et al., 2019, Efficacy of stem cell-based therapies for stroke. Brain Res, 1722: 146362. https://doi.org/10.1016/j.brainres.2019.146362

[33]

Hong H, Yang Y, Zhang Y, et al., 2010, Non-invasive cell tracking in cancer and cancer therapy. Curr Top Med Chem, 10(12): 1237–1248. https://doi.org/10.2174/156802610791384234

[34]

Kircher MF, Gambhir SS, Grimm J, 2011, Noninvasive cell-tracking methods. Nat Rev Clin Oncol, 8(11): 677–688. https://doi.org/10.1038/nrclinonc.2011.141

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