Oxytocin Promotes C6 Glial Cell Death and Aggravates Hydrogen Peroxide-induced Oxidative Stress

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Abstract

Background. Recent studies have shown that oxytocin plays a vital role in neurons and glial cells. However, its effect on hydrogen peroxide (H₂O₂)-induced oxidative stress as well as cyclooxygenase-1 (COX-1) and COX-2 in glial cells are still unclear.
Objective. This study aims to examine the effect of oxytocin on glial cell viability, oxidative stress, COX-1, and COX-2 in C6 glial cells after exposure to H₂O₂.
Methods. In this study, C6 glioma cell line was used to study the effect of oxytocin on the glial cell in four cell groups. The control group was untreated. Cells in the H₂O₂ group were treated with 0.5 mM H₂O₂ for 24 h. Cells in the oxytocin group were treated with various concentrations (0.25, 0.5, 1, and 2 µg/mL) of oxytocin for 24 h. Cells in the oxytocin+H₂O₂ group were pre-treated with various concentrations (0.25, 0.5, 1, and 2 µg/mL) of oxytocin for 1 h before 24-h exposure to 0.5 mM H₂O₂. Cell viability was evaluated using XTT assay. Total antioxidant status and total oxidant status (TOS), COX-1, and COX-2 levels in the cells were measured by commercial kits.
Results. Oxytocin with various concentrations significantly decreased the viability of C6 cells after H₂O₂-induced oxidative stress (P < 0.01). It also significantly increased the levels of TOS, COX-1, and COX-2 in C6 cells after H₂O₂-induced oxidative stress (P < 0.001).
Conclusion. Oxytocin increases glial cell death after H₂O₂-induced oxidative damage in C6 cells, along with upregulation of COX-1 and COX-2 levels.

Keywords

Oxytocin

Oxidative stress

Cell death

Cyclooxygenase-1

Cyclooxygenase-2

C6 glioma

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The authors declare that they have no conflict of interest.

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