AccScience Publishing / GTM / Volume 3 / Issue 2 / DOI: 10.36922/gtm.2568

Hesperetin alleviates pulmonary injury in a blunt chest trauma-induced pulmonary contusion model in rats

Serkan Kaya1 İhsan Karaboğa2* Yasin Duran3 Elif Polat4
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1 Department of Thoracic Surgery, Tekirdağ Dr. İsmail Fehmi Cumalioğlu State Hospital, Tekirdag, Türkiye
2 Department of Histology and Embryology, Kırklareli University, Faculty of Medicine, Kırklareli, Türkiye
3 Department of Pathology Laboratory Techniques, Vocational School of Health Services, Istanbul Rumeli University, Istanbul, Türkiye
4 Department of Histology and Embryology, Faculty of Medicine, Tekirdag Namık Kemal University, Tekirdag, Türkiye
5 Department of General Surgery, Faculty of Medicine, Tekirdag Namık Kemal University, Tekirdag, Türkiye
Global Translational Medicine 2024, 3(2), 2568
Submitted: 29 December 2023 | Accepted: 14 May 2024 | Published: 11 June 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( )

Pulmonary contusion (PC), a condition that occurs frequently in severe thoracic injuries, is a significant contributor to mortality in those under the age of 40. Hesperetin, a natural flavonoid derivative of hesperidin, is a substance found in various citrus fruits such as oranges and grapefruits and possesses a variety of biological activities, including antiapoptotic, antioxidant, and anticancer effects. In the current study, we investigated the effect of hesperetin on pulmonary tissue structure, expression of some pro-inflammatory cytokines, and mediators in a PC model induced by blunt chest trauma. In this study, 18 adult male Wistar albino rats (8 – 10 weeks, 250 – 300 g) were used. The rats were divided into three groups: control, PC, and PC + hesperetin. Hesperetin administration (100 mg/kg/day oral) was completed for 7 days following induction of the model. The wet/dry weight ratio of pulmonary tissue was determined. Tumor necrosis factor alpha (TNFα) and malondialdehyde (MDA) in lung tissues, serum interleukin (IL)-6, and IL-1β levels were determined using the enzyme-linked immunosorbent assays. Pulmonary tissue specimens were examined histologically using hematoxylin-eosin and Masson trichrome staining. Inducible nitric oxide synthase (iNOS) activity was determined using immunohistochemical methods. Hesperetin administration reduced TNFα and iNOS activity, serum IL-1β, IL-6, MDA, and wet/dry weight ratio in pulmonary tissue to improve pulmonary function. Our results showed that administration of hesperetin prevents activation of local inflammatory mediators, thereby obstructing the proinflammatory cytokine cascade and tissue injury.

Tumor necrosis factor alpha
Interleukin 6
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Conflict of interest
The authors declare that they have no competing interests.
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