Novel Strategy for Optimizing the Antibacterial Activity of Psidium guajava Against Clinical Isolates of Escherichia coli, Staphylococcus aureus, Salmonella spp., and Streptococcus spp.
The development of a new antibiotic is a challenging task, with an estimated failure rate of 95%. Minor changes in the chemical structure of a drug, such as stereochemistry, geometry, or functional group modifications, can significantly impact its medicinal activity. In this study, we aim to devise novel strategies for optimizing the antimicrobial properties of guava leaf extract through simple reactions, either by self-reaction or combination reactions with a reagent, drug, or different plant extract. Fourier transform infrared spectroscopy analysis revealed conjugation and formation of new functional groups in the prepared sample of Guava Guava (GG) and Guava Aspirin Guava (GAG), which were further confirmed by weight analysis. The results demonstrated that the antimicrobial activity of medicinal plants can be improved or optimized through simple reactions, such as combining the plant extract with a non-antimicrobial drug like aspirins or adding a small volume of concentrated sulfuric acid to the plant extract by heating at a temperature range of 100 – 110°C. Among the two combinatory methods used, GG exhibited better performance in inhibiting the growth of all tested bacterial strains at a concentration of 0.1 mg/mL compared to GAG at the same concentration, which inhibited the growth of only two bacterial strains: Escherichia coli and Streptococcus spp. These methods can be further explored and applied in various studies, including antifungal, anti-inflammatory, antiviral, and anticancer research, leveraging the availability and diverse range of natural products found in medicinal plants.
[1] Ngene, A.C.; Aguiyi, J.C.; Chibuike, C.J.; Ifeanyi, V.O.; Ukaegbu-Obi, K.M.; Kim, E.G.; Ohaeri, U.C.; Onyemegbulem, B.O. Antibacterial Activity of Psidium guajava Leaf Extract against Selected Pathogenic Bacteria. Adv. Microbiol., 2019, 9, 1012–22.
[2] Rayjade, M.S.; Bhambar, R.S.; Attarde, D.L. A Review on Antimicrobial Activity of Psidium guajava L. Leaves on Different Microbial Species, Antioxidant Activity Profile and Herbal Formulations. J. Pharm. Sci. Res., 2021, 13(7), 406–11.
[3] Yaun, E.A.; Vasquez, B.A. Antibacterial Activity of Formulated Psidium guajava (Guava) Hand Sanitizer Gel on Staphylococcus aureus. J. Res. Univ. Visayas UVJOR, 2017, 11, 1–6.
[4] Díaz-de-Cerio, E.; Verardo, V.; Gómez-Caravaca, A.M.; Fernández-Gutiérrez, A.; Segura-Carretero, A. Health Effects of Psidium guajava L. Leaves: An Overview of the Last Decade. Int. J. Mol. Sci., 2017, 18, 897.
[5] Biswas, B.; Rogers, K.; McLaughlin, F.; Daniels, D.; Yadav, A. Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-negative and Gram-positive Bacteria. Int. J. Microbiol., 2013, 7, 746165.
[6] Khosravi, A.R. Comparison of the Antimicrobial Activity of Garlic Extract with Two Common Antibiotics Against Bacteria Isolated from Urinary Tract Infections. J. Clin. Diagn. Res., 2012, 6(9), 1478–81.
[7] Alzohairy, M.A. Therapeutics Role of Azadirachta indica (Neem) and their Active Constituents in Diseases Prevention and Treatment. Evid. Based Complement. Alternat. Med., 2016, 2016, 7382506.
[8] Jafarnejad, S.; Shaterzadeh, M.J.; Sadegh, F.; Kheiripour, N.; Asghari, G. Antibacterial Activity of Aqueous Extract of Zingiber officinale (Ginger) on Staphylococcus aureus and Escherichia coli. J. Med. Plants Res., 2016, 10(24), 356–61.
[9] Patel, H.M.; Noolvi, M.N.; Sharma, P.; Jaiswal, V.; Bansal, S.; Lohan, S. Quantitative Structure-activity Relationship (QSAR) Studies as Strategic Approach in Drug Discovery. Med. Chem. Res., 2014, 23, 4991–5007.
[10] Prajapat, P.; Vaghani, H.; Agarwal, S.; Talesara, G.L. Synthetic and Medicinal Chemistry in Drug Discovery: Needs for Today. Ann. Med. Chem. Res., 2017, 3(1), 1021.
[11] Veeresham, C. Natural Products Derived from Plants as a Source of Drugs. J. Adv. Pharm. Technol. Res., 2012, 3, 200–1.
[12] Victor, L. Are traditional medicinal plants and ethnobotany still valuable approaches in pharmaceutical research? Bol. Latinoam. Caribb. Plantas Med. Aromát., 2011, 10(1), 3-10.
[13] Fabricant, D.S.; Farnsworth, N.R. The Value of Plants Used in Traditional Medicine for Drug Discovery. Environ. Health Perspect., 2001, 109, 69–75.
[14] Gibbons, S. Anti-staphylococcal Plant Natural Products. Nat. Prod. Rep., 2004, 21(2), 263–277.
[15] Cowan, M.M. Plant Products as Antimicrobial Agents. Clin. Microbiol. Rev., 1999, 12(4), 564–582.
[16] Sneader, W. Drug Discovery: A History. John Wiley and Sons, Hoboken, 2008.
[17] Leung, C.H.; Zhong, H.J.; Ma, D.L. Drug Discovery Targeting Angiogenesis: Anti-angiogenic Principles from Natural Products. Nat. Prod. Rep., 2014, 31(9), 1375–98.
[18] Gideon, M.; Ladan, Z. Synergistic Combinatorial Strategy for Combating Antimicrobial Resistance (AMR) in Clinical Bacteria by Combining Antibiotics with Plant Extracts. Fine Chem. Eng., 2023, 4(1), 1–12.
[19] Gideon, M.; Ladan, Z.; Duniya, E.K.; James, M.A.; Dennis, S. Stimulating Antimicrobial Activity in Aspirin with Psidium guajava and Syzygium aromaticum Extracts Against Multi-drug Resistant Salmonella spp: A Comparative Study of Multiple Combinations. Fine Chem. Eng., 2023, 4(1), 46–57.
[20] Stefanović, O.D. Synergistic activity of antibiotics and bioactive plant extracts: A study against gram-positive and gram-negative bacteria. In: Bacterial Pathogenesis and Antibacterial Control. IntechOpen, London, UK, 2018.
[21] IR Spectrum and Characteristic Absorption Bands. Organic Chemistry I. Available from: https://chem.libretexts.org/ Bookshelves/Organic_Chemistry/Organic_Chemistry_I_ (Liu)/06%3A_Structural_Identification_of_Organic_Compounds-_ IR_and_NMR_Spectroscopy/6.03%3A_IR_Spectrum_and_ Characteristic_Absorption_Bands [Last accessed on 2023 Mar 17].
[22] Lawson, G.; Ogwu, J.; Tanna, S. Quantitative Screening of the Pharmaceutical Ingredient for the Rapid Identification of Substandard and Falsified Medicines Using Reflectance Infrared Spectroscopy. PLoS One, 2018, 13(8), e0202059.
[23] Hassounah, I.A.; Shehata, N.A.; Kimsawatde, G.C.; Hudson, A.G.; Sriranganathan, N.; Joseph, E.G.; Mahajan, R.L. Designing and testing single tablet for tuberculosis treatment through electrospinning. In: Fabrication and Self-assembly of Nanobiomaterials. William Andrew, Norwich, NY, 2016.
[24] Zapata, F.; López-Fernández, A.; Ortega-Ojeda, F.; Quintanilla, G.; Garcia-Ruiz, C.; Montalvo, G. Introducing ATR-FTIR Spectroscopy through Analysis of Acetaminophen Drugs: Practical Lessons for Interdisciplinary and Progressive Learning for Undergraduate Students. J. Chem. Educ., 2021, 98, 2675−86.
[25] Brown, T.L.; LeMay, H.E.; Bursten, B.E.; Murphy, C.J. Chemistry: The Central Science. 14th ed. Pearson Education, Boston, 2017.
[26] Owusu, E.; Ahorlu, M.M.; Afutu, E.; Akumwena, A.; Asare, G.A. Antimicrobial Activity of Selected Medicinal Plants from a Sub- Saharan African Country Against Bacterial Pathogens from Post-operative Wound Infections. Med. Sci. (Basel), 2021, 9, 23.
[27] Ostermeier, C.; Harrenga, A.; Ermler, U. Structure of the Active Center of the Ribonucleotide Reductase from Escherichia coli. Science, 1997, 278(5345), 1663–6.
[28] Pagès, J.M.; Amaral, L. Mechanisms of Drug Efflux and Strategies to Combat them: Challenging the Efflux Pump of Gram-negative Bacteria. Biochim. Biophys. Acta, 2009, 1794(5), 826–33.
[29] Coyne, A.G.; Jones, R.A. Effects of Molecular Structure on the Interaction of Fluoroquinolone Antibacterials with Calcium Phosphate. Chem. Res. Toxicol., 2005, 18(10), 1584–8.
[30] Stereoelectronics. Drug Designe Principles. Stereoelectronics. Available from: https://www.stereoelectronics.org/webdd/dd_04. htmlh_4.1.1 [Last accessed on 2023 Mar 17].