AccScience Publishing / IMO / Online First / DOI: 10.36922/IMO025350042
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ORIGINAL RESEARCH ARTICLE

Effect of Darjeeling black tea aromatics on central nervous system function: An in silico study of glutamate receptor–ligand interactions

Moumita Saha1 ,  Anup Sardar2 ,  Sirshendu Chatterjee1* ,  Anirban Ghosh2*
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1 Department of Biotechnology, Techno India University, Kolkata, West Bengal, India
2 Department of Zoology, Cell Development and Immunobiology Laboratory, School of Sciences, Netaji Subhas Open University, Kalyani, West Bengal, India
IMO, 025350042 https://doi.org/10.36922/IMO025350042
Received: 26 August 2025 | Revised: 14 October 2025 | Accepted: 12 November 2025 | Published online: 5 February 2026
© 2026 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution -Noncommercial 4.0 International License (CC-by the license) ( https://creativecommons.org/licenses/by-nc/4.0/ )
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Abstract

Darjeeling tea (Camellia sinensis var. sinensis) is recognized for its unique aroma and taste, associated with mood and cognitive enhancement. However, the underlying neurochemical mechanisms remain unclear. The present study investigates the potential interaction of Darjeeling tea’s volatile aroma compounds with glutamate receptors (GluRs), the predominant excitatory receptors in the central nervous system (CNS). We hypothesized that these compounds target GluRs to elicit their effects. An in silico approach was employed to analyze the physicochemical properties, bioactivity scores, and toxicity profiles of the aroma compounds. Subsequently, molecular docking simulations were performed using the retrieved 3D structures of relevant GluRs to predict the binding affinity of selected compounds exhibiting high bioactivity, drug-likeness, and bioavailability, and identify key amino acid residues within the receptor binding pockets. Our findings revealed α-ionone and safranal as prominent ligands exhibiting strong binding interactions. Among metabotropic GluRs, mGluR1 (IEWK), GluR5 (3FUZ), and GluR6 (3G3F) showed the highest affinity. Ionotropic receptor subtypes α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (2WJW) and N-methyl-D-aspartate receptor (7EOR) also displayed significant binding scores, where greater structural dynamics were found in metabotropic GluRs on ligand binding compared to ionotropic subtypes. Given that inhalation via the nasal passage is a primary exposure route and the presence of GluR-expressing cells along this pathway, the high bioavailability of α-ionone and safranal suggests their potential to interact with neuroglial cells and subsequently influence CNS neurons and microglia/macrophages. In conclusion, the identified binding of Darjeeling tea’s aromatic ligands and GluRs offers a promising framework for elucidating the mechanisms underlying the tea’s effects on mood, psychological states, and immune-physiological responses.

Graphical abstract
Keywords
Aroma
Darjeeling tea
Glutamate receptors
Molecular docking
Neuro-immune modulation
Funding
None.
Conflict of interest
The authors declare that they have no competing interests.
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Innovative Medicines & Omics, Electronic ISSN: 3060-8740 Print ISSN: 3060-8910, Published by AccScience Publishing
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