AccScience Publishing / IJB / Volume 9 / Issue 5 / DOI: 10.18063/ijb.760
RESEARCH ARTICLE

Antheraea pernyi silk fibroin bioinks for digital light processing 3D printing

Xue Zhang1† Wenbi Wu1† Yulan Huang1 Xiong Yang1 Maling Gou1*
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1 State Key Laboratory of Biotherapy West China Hospital, Sichuan University, Chengdu, Sichuan, China
Submitted: 9 February 2023 | Accepted: 13 March 2023 | Published: 24 May 2023
(This article belongs to the Special Issue 3D Bioprinting with Photocurable Bioinks--Call for Papers )
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
Abstract

The application of three-dimensional (3D) bioprinting has increased in the biomedical field. The lack of bioinks with both biocompatibility and printability is still a problem to be solved. Silk fibroin materials have good biocompatibility and have a broad application prospect in the field of biomedical materials. At present, most research usually involves Bombyx mori silk fibroin (BSF). However, BSF has low cell adhesion. Compared with BSF, Antheraea pernyi silk fibroin (ASF) isolated from typical non-mulberry silk exhibits a unique arginine-glycine-aspartate (RGD) sequence with good cell adhesion enhancement. In this study, we developed a bioink based on ASF for digital light processing (DLP) 3D bioprinting. The ASF-based bioinks (ASF-MA) were produced by a methacryloylation process using methacrylic anhydride (MA) to achieve the properties of photopolymerization reaction. The ASF-MA hydrogel has mechanical properties, biocompatibility, and especially cell adhesion. Meanwhile, we found that the ASF-MA hydrogels promoted the adhesion, migration, and proliferation of S16 cells. Hence, the ASF-MA hydrogels had the potential applications in biomedical fields.

Keywords
Antheraea pernyi silk fibroin
Hydrogels
Photopolymerization
Mechanical properties
Bioprinting
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing