Integrating zinc/silicon dual ions with 3D-printed GelMA hydrogel promotes in situ hair follicle regeneration

Fanliang Zhang^1†, Zhaowenbin Zhang^2†, Xianlan Duan¹, Wei Song¹, Zhao Li¹, Bin Yao¹, Yi Kong¹, Xing Huang³, Xiaobing Fu¹, Jiang Chang^2,4*, Sha Huang^1*

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¹ Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department, PLA General Hospital, Beijing, 100853, China

² Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325000, China

³ Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

⁴ State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, China

†These authors contributed equally to this work.

IJB 2023, 9(3), 703 https://doi.org/10.18063/ijb.703

Received: 25 November 2022 | Accepted: 13 January 2023 | Published online: 8 March 2023

(This article belongs to the Special Issue Integrated Biofabrication Technologies for Constructing Functional Tissue)

© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

The regeneration of hair follicles lost from injury or disease represents a major challenge in cutaneous regenerative medicine. In this study, we investigated the synergetic effects between zinc and silicon ions on dermal cells and screened the optimal concentration of ions for medical applications. We integrated zinc/silicon dual ions into gelatin methacryloyl (GelMA) to bioprint a scaffold and determined that its mechanical properties are suitable for biological treatment. Then, the scaffold was employed to treat mouse excisional model in order to promote in situ hair follicle regeneration. Our findings showed that GelMA-zinc/silicon-printed hydrogel can significantly activate hair follicle stem cells and enhance neovascularization. The beneficial effects of the scaffold were further confirmed by the growth of hairs in the center of wounds and the improvement in perfusion recovery. Taken together, the present study is the first to combine GelMA with zinc/silicon dual ions to bioprint in situ for treating excisional wound, and this approach may regulate hair follicle regeneration not only directly by impacting stem cells but also indirectly through promoting angiogenesis.

Keywords

Zinc and silicon ions

3D bioprinting

GelMA

Hair follicle regeneration

Angiogenesis

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