Ovary-derived Decellularized Extracellular Matrix-based Bioink for Fabricating 3D Primary Ovarian Cells-laden Structures for Mouse Ovarian Failure Correction

Jiahua Zheng¹, Yibin Liu¹, Chenxiao Hou¹, Zhongkang Li¹, Shaopeng Yang², Xiao Liang³, Liang Zhou⁴, Jiangbo Guo⁵, Jingkun Zhang^1*, Xianghua Huang^1*

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¹ Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

² Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

³ Department of Obstetrics and Gynecology, College of Integrated Traditional Chinese and Western Medicine, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China

⁴ Department of Reproductive, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China

⁵ College of Science, Hebei Research Center of Pharmaceutical and Chemical Engineering, Hebei University of Science and Technology, Shijiazhuang, Hebei, China

IJB 2022, 8(3), 597 https://doi.org/10.18063/ijb.v8i3.597

Submitted: 9 March 2022 | Accepted: 29 May 2022 | Published: 26 July 2022

(This article belongs to the Special Issue 3D Printing in Tissue Engineering--Call for Papers )

© 2022 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

Fertility preservation is becoming a clinical duty in practice. Three-dimensional (3D) bioprinting technology is potentially realize ovarian morphological repair and reproductive endocrine function rebuild. There is no published work on 3D bioprinting ovary using a decellularized extracellular matrix (dECM)-based bioink, though dECM is the preferred matrix choice for an artificial ovary. The study aimed to explore swine ovarian dECM-based bioink to fabricate 3D primary ovarian cells (POCs)-laden structures for mouse ovarian failure correction. In this study, the ovarian dECM was converted to dECM-based bioink by dECM solution mixed with a seaweed gelatin blend solution of bioink that was characterized using scanning electron microscopy, circular dichroism, rheology, hematoxylin and eosin staining, and immunohistochemistry. The 3D scaffolds were, then, printed with or without POCs by the extrusion 3D bioprinter. The laden POCs viability was detected with the live/dead assay kit. A female castrated mouse model was established, and the mice were treated with five different methods. The results revealed that the 3D scaffold encapsulating POCs group had more positive signals in neoangiogenesis, cell proliferation and survival than the 3D scaffold group, and ensured sex hormone secretion. Meanwhile, the expression of germ cells in the 3D scaffold encapsulating POCs group was more intensely than the non-printed hydrogel encapsulating POCs group. The work shows that the 3D bioprinting ovary employing ovarian dECM-based bioink is a promising approach for ovarian failure correction.

Keywords

3D bioprinting

Ovary

Decellularized extracellular matrix

Bioink

Primary ovarian cells

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