3D-bioprinted Recombination Structure of Hertwig’s Epithelial Root Sheath Cells and Dental Papilla Cells for Alveolar Bone Regeneration

Huilin Tang^1,2,3, Fei Bi^1,2,3, Guoqing Chen⁴, Shuning Zhang^1,2,3, Yibing Huang^1,2,3, Jiahao Chen^1,2,3, Li Xie^5,6, Xiangchen Qiao⁷, Weihua Guo^1,2,3*

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¹ State Key Laboratory of Oral Disease, West China Hospital of Stomatology, Sichuan University, Chengdu, China

² National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China

³ Department of Pediatric Dentistry, West China School of Stomatology, Sichuan University, Chengdu, China

⁴ Department of Human Anatomy, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China

⁵ National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China

⁶ Engineering Research Center of Oral Translational Medicine, Ministry of Education, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China

⁷ Chengdu Renjitiancheng Biotechnology Limited Corporation, Chengdu, China

IJB 2022, 8(3), 512 https://doi.org/10.18063/ijb.v8i3.512

Submitted: 1 February 2022 | Accepted: 20 April 2022 | Published: 10 June 2022

(This article belongs to the Special Issue 3D Bioprinting with Photocurable Bioinks--Call for Papers )

© 2022 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

Three-dimensional (3D) bioprinting is an emerging method for tissue regeneration. However, promoting the epithelial-mesenchymal interaction (EMI), while maintaining the characteristics of epithelial cells has always been a challenge in tissue engineering. Since EMI acts as a critical factor in bone regeneration, this study aims to promote EMI by recombining epithelial and mesenchymal cells through 3D bioprinting. Hertwig’s epithelial root sheath (HERS) is a transient structure appeared in the process of tooth root formation. Its epithelial characteristics are easy to attenuate under appropriate culture environment. We recombined HERS cells and dental papilla cells (DPCs) through 3D bioprinting to simulate the microenvironment of cell-cell interaction in vivo. HERS cells and DPCs were mixed with gelatin methacrylate (GelMA) separately to prepare bio-inks for bioprinting. The cells/GelMA constructs were transplanted into the alveolar socket of Sprague-Dawley rats and then observed for 8 weeks. Hematoxylin and eosin staining, Masson staining, and immunohistochemical analysis showed that dimensional cultural pattern provided ideal environment for HERS cells and DPCs to generate mineralization texture and promote alveolar bone regeneration through their interactions. 3D bioprinting technology provides a new way for the co-culture of HERS cells and DPCs and this study is inspiring for future research on EMI model.

Keywords

Epithelial-mesenchymal interaction

3D bioprinting

Hertwig’s epithelial root sheath cell

Alveolar bone regeneration

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