Printing amphotericin B on microneedles using matrixassisted pulsed laser evaporation

Roger Sachan¹, Panupong Jaipan², Jennifer Y. Zhang³, Simone Degan³, Detlev Erdmann⁴, Jonathan Tedesco⁵, Lyndsi Vanderwal⁶, Shane J. Stafslien⁶, Irina Negut⁷, Anita Visan⁷, Gabriela Dorcioman⁷, Gabriel Socol⁷, Rodica Cristescu⁷, Douglas B. Chrisey⁸, Roger J. Narayan^2*

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¹ Wake Early College of Health and Sciences, Raleigh, North Carolina, USA

² Joint Department of Biomedical Engineering, The University of North Carolina and North Carolina State University, Raleigh, North Carolina, USA

³ Department of Dermatology, Duke University Medical Center, Durham, North Carolina, USA

⁴ Department of Surgery, Division of Plastic, Reconstructive, Maxillofacial and Oral Surgery, Duke University Medical Center, Durham, North Carolina, USA

⁵ Keyence Corporation of America, Elmwood Park, New Jersey, USA

⁶ Office of Research and Creativity Activity, North Dakota State University, 1715 Research Park Drive, Fargo ND, USA

⁷ National Institute for Lasers, Plasma and Radiation Physics, Lasers Department, P.O. Box MG-36, Bucharest-Magurele, Romania

⁸ Department of Physics and Engineering Physics, Tulane University, New Orleans, LA, USA

IJB 2017, 3(2), 147–157; https://doi.org/10.18063/IJB.2017.02.004

© Invalid date by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

Transdermal delivery of amphotericin B, a pharmacological agent with activity against fungi and parasitic protozoa, is a challenge since amphotericin B exhibits poor solubility in aqueous solutions at physiologic pH values. In this study, we have used a laser-based printing approach known as matrix-assisted pulsed laser evaporation to print amphotericin B on the surfaces of polyglycolic acid microneedles that were prepared using a combination of injection molding and drawing lithography. In a modified agar disk diffusion assay, the amphotericin B-loaded microneedles showed concentrationdependent activity against the yeast Candida albicans. The results of this study suggest that matrix-assisted pulsed laser evaporation may be used to print amphotericin B and other drugs that have complex solubility issues on the surfaces of microneedles.

Keywords

matrix-assisted pulsed laser evaporation

microneedle

amphotericin B

antifungal

References

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