Phytoremediation of Domestic Sewage in Constructed Wetland Integrated with Cultivation of Chlorella sp.: A Novel Technique for Remediation and Resource Recovery

Domestic sewage-based constructed wetland (CW) showed that the effluent from CW-system contain enough plant nutrients and enhanced the growth of microalgae. Hence, a pilot CW system employing Typha latifolia in domestic sewage, integrated with the cultivation wild type Chlorella sp. was investigated. Phytoremediation at 48 hours of detention time caused significant changes in its physicochemical properties and the generated effluent was notably attractive for the cultivation of microalgae. The microalga was grown in 6 treatments: treated-mixotrophic (T1), treated-heterotrophic (T2), treated-autotrophic (T3), control-mixotrophic (T4), controlheterotrophic (T5) and control-autotrophic (T6) conditions for 8 days inside an incubator. The results suggested that phytoremediation effluents integrated with mixotrophic cultivation of microalgae, utilising both light and carbon sources could be the most efficient, environmentally safe, sustainable and novel technique for synergistic resource generation and bioremediation.
Allen, S.E. (1998). Chemical analysis of ecological materials, Second (ed.) Blackwell Scientific Publication. Butler & Tanner Ltd., Rome and London, 1998.
American Public Health Association (2006). Standard methods for the examination of water and wastewater. Water Environment Federation, Washington, DC.
Cheirslip, B. and S. Torpee (2012). Enhanced growth and lipid production of microalgae under mixotrophic culture condition: Effect of light intensity, glucose concentration and fed-batch cultivation. Bioresource Technology, 102: 17-25.
Gao, K., Liu, Q., Gao, Z., Xue, C., Qian, P., Dong, J., Gao, Z. and X. Deng (2021). A dilution strategy used to enhance nutrient removal and biomass production of Chlorella sorokiniana in frigon wastewater. Algal Research, 58: 102428.
Garcia, O.P., de-Bashan, L.E., Hernandez, J. and Y. Bashan (2010). Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense., J. Phycol., 46: 800-812.
Giri, A.K., Sacchan, P., Kushwaha, S., Singh, M.P. and S.Verma (2012). Increasing nitrogen uptake and removal efficiency of Eichhornia crassipes from domestic sewage through dilution culture study. Asian Journal of Water, Environment and Pollution, 9: 45-50..
Gomez, K.A. and A.A. Gomez (1984). Statistical procedure for agricultural research, John Wiley, New York.
Gour, R.S., Kant, A. and R.S. Chouhan (2014). Screening of microalgae for growth and lipid accumulation properties. J. Algal. Biomass Utln., 5: 38-46.
Greenwell, H.C., Laurens, L.M.L., Shields, R.J., Lovitt, R.W. and K.J. Flynn (2010). Placing microalgae on the biofuels priority list: A review of the technological challenges. J. R. Soc., Interface, 7: 703-726.
Grobbelaar, J.U. (2004). Algal nutrition, mineral nutrition. In: Richmond, A. (Ed.) Handbook of microalgal culture. Biotechnology and Applied Phycology, Blackwell Publishing, Oxford, UK, pp. 97-115.
Juwarker, A.S., Oke, B., Juwarker, A. and S.M. Pataink (1995). Domestic wastewater treatment through constructed wetland in India. Wat. Sci. Tech., 32: 291-294.
Kadlec, H.R. and R.L. Knight (1996). Treatment Wetlands, Lewis, Boca Raton, New York, London, Tokyo.
Kivaisi, A.K. (2001). The potential for constructed wetlands for wastewater treatment and reuse in developing countries: A review. Ecological Engineering, 16: 545-560.
Lin, Q., Gu, N., Li, G., Lin, J., Huang, L. and L.Tan (2012). Effects of inorganic carbon concentration on carbon formation, nitrate utilization, biomass and oil accumulation of Nannochloropsis oculata CS 179. Bioresource Technology, 111: 353-359.
Mitra, D., van Leeuwen, J. and B. Lamsal (2012). Heterotrophic/mixotrophic cultivation of oleaginous Chlorella vulgaris on industrial co-products. Algal Research, 1: 40-48.
Park, J.B.K., Craggs, R.J. and A.N. Shilton (2011). Wastewater treatment high rate algal ponds for biofuel production. Bioresource Technology, 102: 5-42.
Pittman, J.K., Dean, A.P. and O. Osundeko (2011). The potential of sustainable algal biofuel production using wastewater resources. Bioresource Technology, 102: 17- 25.
Wang, L., Min, M., Li, Y., Chen, P., Chen, Y., Liu, Y., Wang, Y. and R. Ruan (2010). Cultivation of green algae chlorella sp. in different wastewaters from municipal wastewater treatment plant. Appl. Biochem. Biotechnol., 162: 1174-1186.
Yang, C., Hua, Q. and K. Shimizu (2000). Energetics and carbon metabolism during growth of microalgal cells under photoautotrophic, mixotrophic and cyclic lightautotrophic⁄dark-heterotrophic conditions. Biochem. Eng. J., 6: 87-102.