AccScience Publishing / IJB / Online First / DOI: 10.36922/ijb.1806
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RESEARCH ARTICLE

Biomimetic mineralization of 3D-printed polyhydroxyalkanoate-based microbial scaffolds for bone tissue engineering

Dahong Kim1,2 ,  Su Jeong Lee3 ,  Dongjin Lee1 ,  Ji Min Seok1,2 ,  Seon Ju Yeo1 ,  Hyungjun Lim1 ,  Jae Jong Lee1 ,  Jae Hwang Song4 ,  Kangwon Lee2,5 ,  Won Ho Park6 ,  Su A Park1*
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1 Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon, Republic of Korea
2 Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea
3 Department of Microbiology, CHA University, Seongnam, Republic of Korea
4 Department of Orthopaedic Surgery, Konyang University Hospital, Daejeon, Republic of Korea
5 Research Institute for Convergence Science, Seoul National University, Seoul, Republic of Korea
6 Department of Organic Materials Engineering, Chungnam National University, Daejeon, Republic of Korea
IJB 2024, 10(2), 1806 https://doi.org/10.36922/ijb.1806
Submitted: 12 September 2023 | Accepted: 2 November 2023 | Published: 16 January 2024
© 2024 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

Polyhydroxyalkanoates (PHAs) have gained much attention as a potential alternative to conventional plastic bone scaffolds due to their biocompatibility and biodegradability, among a diverse range of advantageous properties. However, the water resistance of PHA creates an environment that can interfere with cell interactions. In this study, a three-dimensional-printed PHA scaffold was fabricated through fused deposition modeling printing considering the physical properties of PHA. The PHA bone scaffolds were then coated with polydopamine (pDA) and/or hydroxyapatite (HA) in various configurations using a relatively simple and rapid process involving only immersion. The PHA–pDA– HA scaffold showed enhanced cell viability, proliferation, and differentiation, and could thus serve as a versatile platform for bone tissue engineering applications.

Keywords
Polyhydroxyalkanoate
Biomineralization
Biopolymer
Bone scaffold
Polydopamine
Bioprinting
Funding
This research was supported by the National Research Foundation (NRF; No. NRF-2019M3A9E2066348 and NRF-2021M3H4A4079292) and the National Research Council of Science & Technology (CRC22021-200) funded by the Korean government.
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Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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