Matrix-Assisted Pulsed laser Evaporation-deposited Rapamycin Thin Films Maintain Antiproliferative Activity

Rodica Cristescu¹, Irina Negut¹, Anita Ioana Visan¹, Alexander K. Nguyen^2,4, Andrew Sachan³, Peter L. Goering⁴, Douglas B. Chrisey⁵, Roger J. Narayan^2*

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¹ Department of Lasers, National Institute for Lasers, Plasma and Radiation Physics, P.O. Box MG-36, Bucharest-Magurele, Romania

² UNC/NCSU Joint Department of Biomedical Engineering, Raleigh, North Carolina, USA

³ Wake Technical Community College, Raleigh, North Carolina, USA

⁴ Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, Maryland, United States

⁵ Department of Physics and Engineering Physics, Tulane University, New Orleans, LA, USA

IJB 2020, 6(1), 188 https://doi.org/10.18063/ijb.v6i1.188

Published: 30 January 2020

© 2020 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

Matrix-assisted pulsed laser evaporation (MAPLE) has many benefits over conventional methods (e.g., dip-coating, spin coating, and Langmuir–Blodgett dip-coating) for manufacturing coatings containing pharmacologic agents on medical devices. In particular, the thickness of the coating that is applied to the surface of the medical device can be tightly controlled. In this study, MAPLE was used to deposit rapamycin-polyvinylpyrrolidone (rapamycin-PVP) thin films onto silicon and borosilicate optical glass substrates. Alamar Blue and PicoGreen studies were used to measure the metabolic health and DNA content of L929 mouse fibroblasts as measures of viability and proliferation, respectively. The cells on the MAPLE-deposited rapamycin-PVP surfaces exhibited 70.6% viability and 53.7% proliferation compared to a borosilicate glass control. These data indicate that the antiproliferative properties of rapamycin were maintained after MAPLE deposition.

Keywords

Rapamycin

Drug delivery

Matrix-assisted pulsed laser evaporation

Thin film

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