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Cyclin-Dependent Kinase 8 (CDK8) Immunoexpression in Uveal Melanoma

Daniel M. Real1,2 Edina A. Wappler-Guzzetta1,3 Chelsea Huang1 Aleksander M. Real4 Jeremy K. Deisch1 Justin C. Kerstetter1*
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1 Department of Pathology, Loma Linda University Medical Center, Loma Linda, CA 92354, USA
2 Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA
3 Department of Laboratory Medicine, University of California San Francisco, San Francisco, CA 94143, USA
4 Loma Linda University School of Medicine, Loma Linda, CA 92354, USA
CP 2023, 5(4), 2608
Submitted: 20 October 2023 | Accepted: 18 December 2023 | Published: 31 December 2023
© 2023 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( )

Background: Cyclin-dependent kinase 8 (CKD8) is a component of the mediator complex, which regulates the gene transcription of nearly all RNA polymerase II-dependent genes. It is known to be overexpressed in more aggressive cutaneous melanomas, but no data exist for its uveal counterpart. Methods: Retrospective study on human UM tissue, performing CDK8 immunostaining (n=19). For analysis, a scale of 0-3 staining intensity was used along with an extent score, the percentage of positively staining cells (0-100%), and the confirmed pathological stages.  Results: No significant change was seen in the CDK8 staining patterns between the different pathologic stages, but there was a trend of higher pathological stage showing lower staining intensity score between the stages (p=0.06). Conclusions: Lower CDK8 immunoreactivity was associated with a trend for higher grade UM, highlighting the possible tumor suppressor role of CDK8 in UM advancement.

Uveal melanoma
Ocular melanoma
Cyclin-dependent kinase 8 immunoexpression
Ocular oncology
No external funding or other financial support received.
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Conflict of interest
The authors declare they have no competing interests.
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