Understanding the responsiveness of nitric oxide to acute eccentric resistance exercise in elderly obese women
Background and Aim: The present study examines nitrite concentration responses following eccentric resistance exercise (ERE) in elderly obese women. The existence of high (HR) and low responders (LR) for nitrite and the possible differences with respect to creatine kinase (CK) response, metabolic and body composition variables were also investigated.
Methods: Forty-nine elderly obese women completed an ERE session utilizing knee extensor exercise. LR for serum nitrite were defined as a ∆nitrite ≤ 20th percentile or 24.1 µM and HR as a ∆ nitrite > 20th percentile.
Results: Ten subjects were classified as LR and the remaining as HR (n = 39). The HR group displayed greater nitrite concentration at 0 h, 3 h, 24 h and 48 h following the ERE as compared with the LR (p < 0.05), and CK increased after 24 h and 48 h only for the HR group following the ERE (p < 0.05). Peak nitrite concentration was higher in the HR group versus the LR group (p < 0.05), while there was no difference between groups for pre-exercise nitrite values. The LR group displayed higher (p < 0.05) body fat, cholesterol, LDL and lower upper limb fat-free mass as compared with the HR group. The LR had lower (p < 0.05) upper limb fat-free mass than the HR group.
Conclusions: Elderly obese women classified as HR displayed higher nitrite responses to ERE. Thus, re-searchers should be aware of the presence of different responsiveness of nitrite to acute exercise to avoid misinterpretation of data and to identify the higher cardiovascular risk factor of those classified as LR.
Relevance for patients: The elevated NO up to 48 h following an ERE session may suggest an important protective cardiovascular effect. The higher body fat, cholesterol, LDL and lower upper limb fat-free mass in the LR group might represent a deleterious effect of lower serum levels of nitrite.
[1] Mathus-Vliegen EM. Obesity and the elderly. J Clin Gastroenterol. 2012; 46: 533-544.
[2] Baker BA, Cutlip RG. Skeletal muscle injury versus adaptation with aging: novel insights on perplexing paradigms. Exerc Sport Sci Rev. 2010; 38: 10-16.
[3] Mayhan WG, Arrick DM, Sharpe GM, Sun H. Age-related alterations in reactivity of cerebral arterioles: role of oxidative stress. Microcirculation. 2008; 15: 225-236.
[4] Jin RC, Loscalzo J. Vascular Nitric Oxide: Formation and Function. J Blood Med. 2010; 2010: 147-62.
[5] Zahedi Asl S, Ghasemi A, Azizi F. Serum nitric oxide metabolites in subjects with metabolic syndrome. Clin Biochem. 2008; 41: 1342-1347.
[6] Franzoni F, Galetta F, Morizzo C, Lubrano V, Palombo C, Santoro G, Ferrannini E, Quiñones-Galvan A. Effects of age and physical fitness on microcirculatory function. Clin Sci (Lond). 2004; 106: 329-335.
[7] Lima PM, Santiago HP, Szawka RE, Coimbra CC. Central blockade of nitric oxide transmission impairs exercise-induced neuronal activation in the PVN and reduces physical performance. Brain Res Bull. 2014; 108: 80-87.
[8] Zdrenghea D, Bodizs G, Ober MC, Ilea M. Plasma nitric oxide metabolite levels increase during successive exercise stress testing - A link to delayed ischemic preconditioning? Exp Clin Cardiol. 2003; 8: 26-28.
[9] Bouchard C, Blair SN, Church TS, Earnest CP, Hagberg JM, Hakkinen K, Jenkins NT, Karavirta L, Kraus WE, Leon AS, Rao DC, Sarzynski MA, Skinner JS, Slentz CA, Rankinen T. Adverse metabolic response to regular exercise: is it a rare or common occurrence? PLoS One. 2012; 7: e37887.
[10] Roig M, Macintyre DL, Eng JJ, Narici MV, Maganaris CN, Reid WD. Preservation of eccentric strength in older adults: Evidence, mechanisms and implications for training and rehabilitation. Exp Gerontol. 2010; 45: 400-409.
[11] Tajra V, Tibana RA, Vieira DC, de Farias DL, Teixeira TG, Funghetto SS, Silva AO, de Sousa NM, Willardson J, Karnikowski MG, Prestes J. Identification of high responders for interleukin-6 and creatine kinase following acute eccentric resistance exercise in elderly obese women. J Sci Med Sport. 2014; 17: 662-666.
[12] Clarkson PM, Ebbeling C. Investigation of serum creatine kinase variability after muscle-damaging exercise. Clin Sci (Lond). 1988;75:257-261.
[13] Kim J, Lee J. The relationship of creatine kinase variability with body composition and muscle damage markers following eccentric muscle contractions. J Exerc Nutrition Biochem. 2015; 19: 123-129.
[14] Clarkson PM, Apple FS, Byrnes WC, McCormick KM, Triffletti P. Creatine kinase isoforms following isometric exercise. Muscle Nerve. 1987; 10: 41-44.
[15] Newton MJ, Morgan GT, Sacco P, Chapman DW, Nosaka K. Comparison of responses to strenuous eccentric exercise of the elbow flexors between resistance-trained and untrained men. J Strength Cond Res. 2008; 22: 597-607.
[16] Clarkson PM, Hoffman EP, Zambraski E, Gordish-Dressman H, Kearns A, Hubal M, Harmon B, Devaney JM. ACTN3 and MLCK genotype associations with exertional muscle damage. J Appl Physiol (1985). 2005; 99: 564-569.
[17] da Cunha Nascimento D, de Sousa NM, de Sousa Neto IV, Tibana RA, de Souza VC, Vieira DC, Camarço NF, de Oliveira S, de Almeida JA, Navalta J, Prestes J. Classification of pro-inflammatory status for interleukin-6 affects relative muscle strength in obese elderly women. Aging Clin Exp Res. 2015; 27: 791-797.
[18] Rodriguez-Miguelez P, Lima-Cabello E, Martinez-Florez S, Almar M, Cuevas MJ, Gonzalez-Gallego J. Hypoxia-inducible factor-1 modulates the expression of vascular endothelialgrowth factor and endothelial nitric oxide synthase induced by eccentric exercise. J Appl Physiol (1985). 2015; 118: 1075- 1083.
[19] Mayhan WG, Arrick DM, Sharpe GM, Sun H. Age-related alterations in reactivity of cerebral arterioles: role of oxidative stress. Microcirculation. 2008; 15: 225-236.
[20] Nascimento Dda C, Navalta JW, Durigan JL, Marqueti Rde C, Tibana RA, Luiz Franco O, de Almeida JA, Camarço NF, Neto IV, Prestes J. Acute eccentric resistance exercise decreases matrix metalloproteinase activity in obese elderly women. Clin Physiol Funct Imaging. 2016; 36: 139-145.
[21] Machado M, Willardson JM. Short recovery augments magnitude of muscle damage in high responders. Med Sci Sports Exerc. 2010; 42: 1370-1374.
[22] Santana HA, Moreira SR, Neto WB, Silva CB, Sales MM, Oliveira VN, Asano RY, Espíndola FS, Nóbrega OT, Campbell CS, Simões HG. The higher exercise intensity and the presence of allele I of ACE gene elicit a higher post-exercise blood pressure reduction and nitric oxide release in elderly women: an experimental study. BMC Cardiovasc Disord. 2011; 11: 71.
[23] Funghetto SS, Prestes J, Silva Ade O, Farias DL, Teixeira TG, Vieira DC, Souza VC, Sousa NM, Navalta JW, Melo GF, Karnikowski MG. Interleukin-6 -174 G/C gene polymorphism affects muscle damage response to acute eccentric resistance exercise in elderly obese women. Exp Gerontol. 2013; 48: 1255-1259
[24] Kortebein P, Ferrando A, Lombeida J, Wolfe R, Evans WJ. Effect of 10 days of bed rest on skeletal muscle in healthy older adults. JAMA. 2007; 297: 1772-1774.
[25] Olszanecka-Glinianowicz M. BZ-M, M.Plewa, J.Janowska. The effect of short – term exercise on nitric oxide (NO) serum concentrations in overweight and obese women. Biol Sport. 2008; 25: 125-134.
[26] Chen TC. Effects of a second bout of maximal eccentric exercise on muscle damage and electromyographic activity. Eur J Appl Physiol. 2003; 89: 115-121.
[27] Rigamonti E, Touvier T, Clementi E, Manfredi AA, Brunelli S, Rovere-Querini P. Requirement of inducible nitric oxide synthase for skeletal muscle regeneration after acute damage. J Immunol. 2013; 190: 1767-1777.
[28] Teixeira TG, Tibana RA, Nascimento DD, de Sousa NM, de Souza VC, Vieira DC, Nóbrega OT, de Almeida JA, Navalta J, Prestes J. Endothelial nitric oxide synthase Glu298Asp gene polymorphism influences body composition and biochemical parameters but not the nitric oxide response to eccentric resistance exercise in elderly obese women. Clin Physiol Funct Imaging. 2015; Jun 5.
[29] Mann TN, Lamberts RP, Lambert MI. High responders and low responders: factors associated with individual variation in response to standardized training. Sports Med. 2014; 44: 1113-1124.
[30] Roig M, Macintyre DL, Eng JJ, Narici MV, Maganaris CN, Reid WD. Preservation of eccentric strength in older adults: Evidence, mechanisms and implications for training and rehabilitation. Exp Gerontol. 2010; 45: 400-409.
[31] Heled Y, Bloom MS, Wu TJ, Stephens Q, Deuster PA. CK-MM and ACE genotypes and physiological prediction of the creatine kinase response to exercise. J Appl Physiol (1985). 2007; 103: 504-510.