Three-dimensional (3D) bioprinting has emerged as a powerful platform for fabricating complex biological constructs. Nevertheless, significant challenges remain in translating biomaterial design into functional tissue outcomes. This special issue aims to highlight recent advances in biofabrication strategies that bridge the gap between biomaterials development and biological performance. Emphasis will be placed on the rational design of bioinks, cell–material interactions, and the maturation of bioprinted constructs toward functional tissue development. Contributions addressing innovative biomaterials, multi-material and hybrid fabrication approaches, and strategies to enhance structural, mechanical, and biological functionality are particularly encouraged. By integrating perspectives from biomaterials science, tissue engineering, and bioprinting technologies, this issue aims to provide a comprehensive overview of current progress and emerging directions toward clinically relevant and biologically functional engineered tissues.