This special issue focuses on Advanced Functional Bio-inks for 3D Bioprinting: From Material Design to Translational Tissue Engineering Applications, covering functional material design at the molecular level, rheological and mechanical regulation of multicomponent bio-inks, functional modification, as well as tissue engineering construction toward clinical translation. The scope includes stimuli-responsive bio-inks and the development of bio-inks for specific tissues, such as vascularization, nerve regeneration, and osteochondral repair. This special issue also highlights the synergistic optimization of printability and cell viability of bio-inks, multi-material and multi-cell loading strategies, the design and standardization of biodegradable, fatigue-resistant, and in-situ crosslinkable bio-inks for clinical translation, as well as the functional validation and translational pathway exploration of bio-inks in in vitro and in vivo models.

By bringing together complementary innovations in materials science, cell biology, and biomanufacturing, this special issue aims to provide a systematic and forward-looking vision for the fundamental research, technological breakthroughs, and clinical tissue engineering applications of advanced functional bio-inks.