AccScience Publishing / IJB / Volume 10 / Issue 2 / DOI: 10.36922/ijb.2323
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RESEARCH ARTICLE

Roles of pore architecture of artificial bone grafts in invasion competition between bone and fibrous tissue and orientation of regenerated bone

Keigo Shibahara1,2 ,  Koichiro Hayashi1* ,  Yasuharu Nakashima2 ,  Kunio Ishikawa1
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1 Department of Biomaterials Faculty of Dental Science, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan
2 Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan
IJB 2024, 10(2), 2323 https://doi.org/10.36922/ijb.2323
Submitted: 28 November 2023 | Accepted: 22 January 2024 | Published: 1 March 2024
(This article belongs to the Special Issue Advanced Biomaterials for 3D Printing and Healthcare Application)
© 2024 by the Author(s).. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )
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Abstract

 In the reconstruction of bone defects close to soft tissue, preventing the invasion of fibrous tissue into the bone defect is key to successful bone reconstruction. In this study, we clarified the effects of the pore architecture of artificial bone grafts on the penetration of bone and fibrous tissue, and the orientation of regenerated bone. Carbonate apatite grafts with uniaxial pores along the long- (L-graft) or short-axis (S-graft) direction of the graft and biaxial pores along the long- and short-axes (LS-graft) were used. These grafts were implanted in bone defects created by rabbit ulnae amputation. The pores of the L-, S-, and LS-grafts opened into the bone stumps, muscles, and bone stumps and muscles together, respectively. In the L-graft, the graft pores developed bone from the bone stump to the graft center, while preventing excessive invasion of fibrous tissue. In S- and LS-grafts, the graft pores along the short axis allowed the invasion of fibrous tissue into the grafts. Consequently, although the bone grew to the edge regions in these grafts, further bone ingrowth was inhibited by the fibrous tissue. Furthermore, the pore architecture of the grafts affected the orientation of the regenerated bone. The degree of orientation of the bone formed in the L- and S-grafts was 1.6-fold higher than that formed in the LS-grafts. Thus, controlling the pore architecture allowed the growth of bone to predominate over that of fibrous tissue and induced the formation of bone with an ordered orientation

Keywords
Pore architecture
Bone
Apatite
Tissue regeneration
Graft
Funding
This study was supported in part by Japan Agency for Medical Research and Development under grant no. JP22ym0126098h and Japan Society for the Promotion of Science KAKENHI under grant nos. JP23K18593 and JP22H03954.
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Conflict of interest
The authors declare no conflicts of interest.
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International Journal of Bioprinting, Electronic ISSN: 2424-8002 Print ISSN: 2424-7723, Published by AccScience Publishing
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