LncRNA and Gene expression profiling of human bladder cancer

Zhao Yang^1*, Haifeng Wang^2*, Suhang Bai¹, Zongyi Shen¹, Zhong Bo¹, Qiuyun Yan¹, Dongxiao Cheng¹, Wei Zhang⁴, Jian Zhuang⁵, Lingzhi Wang¹, Xinlu Yu¹, Fuhan Zhang¹, Ruonan Gao¹, Yuhan Yan⁶, Changyuan Yu^1#, Chong Li^3#

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¹ Department of Biomedical Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

² Department of Urology, Second Affiliated Hospital of Kunming Medical University, Kunming 650 101, China

³ Core Facility for Protein Research, Institute of Biophysics, Chinese Academy of Sciences, Beijing 10010, China

⁴ Department of Urology, Affiliated Hospital of Hebei University, Baoding 071 030, China

⁵ Institute of Plastic Machinery and Engineering, College of Mechanical and Electrical Engineering, Beijing University of Chemical Technology, Beijing 100029, China

⁶ Department of Management Science and Engineering, School of Business, Renmin University of China, Beijing 100872, China

CP 2019, 1(1), 43–49; https://doi.org/10.18063/cp.v1i1.221

Received: 7 March 2019 | Accepted: 14 March 2019 | Published online: 19 March 2019

© 2019 by the Author(s). This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ )

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Abstract

Bladder cancer (BC) is the most common urologic neoplasms with a high rate of recurrence. The formation of BC is a complicated process, resulting from smoke, drugs, or bladder inflammation. However, the underlying mechanisms of BC tumorigenesis remained unclear. In this study, we performed microarray analysis of three paired paratumor (PT) and tumor tissues from BC patients. In comparison with PT, BC tissues displayed 1136 upregulated and 1199 downregulated lncRNAs, and 1347 upregulated and 953 downregulated mRNAs. In addition, the upregulated mRNAs were focused in SNARE interactions, amino sugar and nucleotide sugar metabolism, and antigen processing pathways. The common downregulated genes were mainly focused in amino acid metabolism and drug metabolism signaling. More importantly, cytokinerelated genes were highly expressed in BC, which suggest that the dysregulation of immunoregulatory process and amino acid metabolism played a crucial role in the oncogenesis of BC.

Keywords

amino acid metabolism

bladder cancer

cytokine signaling pathway

lncRNA

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